Magungunan peptide gabaɗaya ana bayyana su azaman polymers waɗanda suka ƙunshi haɗin haɗin amide tare da ragowar amino acid ƙasa da 40.Saboda babban aikin mai karɓa da zaɓin magungunan peptide tare da ƙananan haɗarin sakamako masu illa, an sami sha'awa mai ƙarfi ga peptides daga masana'antar harhada magunguna.A wannan lokacin, akwai kuma magungunan taurari da yawa, waɗanda aka fi mayar da hankali a cikin masana'antar cututtukan rayuwa, kamar GLP-1 analogue somalutide, peptide inhibitory peptide (GIP) glucagon-kamar peptide-1 (GLP-1) tesiparatide da sauran dual. - agonists masu karɓa.Bugu da ƙari, tare da haɓakar magungunan PDC da RDC.A halin yanzu, hanyoyin shirye-shiryen magungunan polypeptide sun haɗa da haɗakar sinadarai da fermentation na halitta.Ana amfani da biofermentation galibi don samar da dogon peptides.Abubuwan da ake amfani da su sune ƙananan farashin samarwa, amma rashin iya gabatar da amino acid marasa kyau a cikin jerin peptide da rashin iya yin kayan ado daban-daban akan sarkar peptide.Saboda haka, aikace-aikacen sa kuma yana da iyaka sosai.Hanyoyin haɗin sinadarai sun haɗa da m lokaci kira da ruwa lokaci kira.Ƙaƙƙarfan kira mai ƙarfi yana da fa'ida mai mahimmanci akan haɗakar ruwa-lokaci: za'a iya amfani da wuce haddi na abu don amsawa don tabbatar da cikakken haɗin gwiwa.Ana iya cire amino acid da suka wuce kima, wakilai masu raguwa, da samfuran samfuran ta hanyar ayyuka masu sauƙi na tsaftacewa, guje wa hadaddun sarrafawa da ayyukan tsarkakewa da inganta ingantaccen aiki, don haka hanyar haɗaɗɗiyar lokaci mai ƙarfi ta kasance mafi yawan amfani."Kayan kayan aikin sunadarai don haɗin peptides sun haɗa da kayan farawa, reagents, da kaushi."Ingancin su, musamman ingancin kayan farko, na iya yin tasiri daban-daban akan ingancin API.Abubuwan da aka fara farawa galibi suna nufin abubuwan da aka samo asali na amino acid da aka tabbatar don sarkar peptide da aka gyara fatty acids, polyethylene glycol, da sauransu. A matsayin mahimman gutsuttsuran tsarin, an rarraba su azaman kayan cikin tsarin API, wanda ke da alaƙa kai tsaye da ingancin API.Don haka, ya kamata mu mai da hankali kan sarrafa kayan farawa.
I. Sanya zaɓin kayan farko
ICHQ11 yana ba da shawara a sarari cewa idan ana amfani da samfurin sinadari da aka sayar a kasuwa azaman ɗanyen farko, mai nema ba ya buƙatar ya tattauna dalilinsa.Abubuwan sinadarai da ake sayarwa a kasuwa gabaɗaya ana iya amfani da su ba kawai azaman kayan farawa don magunguna ba, har ma ana iya siyar da su a kasuwannin da ba na magunguna ba.Abubuwan da aka keɓance da haɗaka ba su cikin samfuran sinadarai da aka sayar a kasuwa.Ko da yake babu kasuwar da ba ta magani ba don kare amino acid don saduwa da ma'anar ICHQ11 na sinadarai da ake sayar da su a kasuwa, suna da ƙayyadaddun, bambanta da sinadarai kuma a bayyane, suna da sauƙin ware da tsarkakewa, kuma ana iya gano su da gwada su ta hanyar bincike na yau da kullum. .Suna da ingantaccen kaddarorin sinadarai kuma suna da sauƙin adanawa, jigilar kayayyaki, da haɗa su
Ii.Sarrafa abubuwan da suka dace a cikin kayan farawa
Amino acid ɗin kariya da aka ambata an haɗa su cikin tsarin API a matsayin muhimmin sashi na tsari, wanda ke da alaƙa kai tsaye da ingancin API ɗin.Saboda haka, ya kamata mu kula sosai da abubuwan da ke cikin ƙazanta a cikin kayan farko, mu fahimci canji da kau da waɗannan ƙazanta a cikin tsarin da aka kafa, kuma a ƙarshe mu bayyana dangantakar da ke tsakanin su da ƙazantattun abubuwan da ke cikin API.
Fahimtar kayan farawa da magungunan polypeptide
Na uku, ragowar sauran ƙarfi a cikin kayan farko
Gabaɗaya, idan aka yi la’akari da ƙayyadaddun ƙayyadaddun ƙaƙƙarfan ƙarni na peptides, za a yi amfani da babban adadin ƙarfi don tsabtace guduro peptide bayan kammala kowane mataki na haɗin gwiwar amino acid da karewa daga kariya.Danyen peptides da aka samu ta hanyar fasa resin peptide shima za a yi ta HPLC kuma a bushe daskare.Don haka, akwai ɗan haɗari cewa ƙaramin adadin ƙarfi da ke haɗe da amino acid masu kariya za a isar da su zuwa API na ƙarshe.Duk da haka, ya kamata a ba da kulawa ta musamman ga ragowar acetate, butyl acetate, da kuma barasa, saboda waɗannan kaushi na iya haifar da lahani tare da amino acid masu aiki ko sarƙoƙi na peptide a lokacin haɗin gwiwar amino acid.Misali, yayin hada-hadar amino acid, ragowar acetic acid zai amsa tare da rukunin amino da aka fallasa akan sarkar peptide, wanda zai haifar da rufaffiyar sarkar peptide;A lokacin aikin amino acid, ragowar barasa na iya amsawa tare da rukunin carboxyl mai aiki, wanda zai haifar da wucewar amino acid mai aiki, rage daidai da amino acid, kuma a ƙarshe yana haifar da ƙarancin haɗin amino acid da rashin ƙarancin peptide.Kamfanin yana sarrafa butyl acetate, barasa, methanol, da acetic acid a cikin COA, yana ɗaukar amino acid daga Zheng Yuan Biochemical a matsayin misali.Ma'auni na butyl acetate shine ≤0.5% butyl acetate, wanda a zahiri aka gano ya zama 0.10%.A cewar ICHQ3C, butyl acetate don nau'ikan kaushi guda uku, ya kafa ma'auni na 0.5% ko ƙasa da haka daidai da buƙatun ICHQ3C, amma la'akari da butyl acetate amino acetylation na iya haifar da haɗari, kuma yana hulɗa da butyl acetate don daidaita bincike. , don ƙayyade ma'auni mafi dacewa.
Lokacin aikawa: Agusta-29-2023