A matsayin babban madaidaicin kayan aiki, HPLC na iya haifar da wasu ƙananan matsalolin cikin sauƙi idan ba a sarrafa shi ta hanyar da ta dace yayin amfani.Daya daga cikin matsalolin gama gari shine matsalar matsawa shafi.Yadda ake saurin magance chromatograph mara kyau.Tsarin HPLC ya ƙunshi kwalban tafki, famfo, injector, ginshiƙi, ɗakin zafin jiki, na'urar ganowa da tsarin sarrafa bayanai.Ga dukkan tsarin, ginshiƙai, famfo da masu ganowa sune mahimman abubuwan da aka gyara da kuma manyan wuraren da ke da matsala.
Makullin matsa lamba shafi shine yankin da ke buƙatar kulawa sosai lokacin amfani da HPLC.Kwanciyar kwanciyar hankali na ginshiƙi yana da alaƙa da alaƙa da siffar kololuwar chromatographic, ingancin shafi, ingantaccen rabuwa da lokacin riƙewa.Kwanciyar ginshiƙi ba yana nufin cewa ƙimar matsa lamba ta tsaya tsayin daka a madaidaicin ƙima, amma a maimakon haka madaidaicin matsa lamba yana tsakanin 345kPa ko 50PSI (ba da damar yin amfani da gradient elution lokacin da matsi na ginshiƙi ya tabbata kuma a hankali yana canzawa).Matsayi mai girma ko ƙarancin ƙarfi shine matsalar matsa lamba.
Mafi saukin kamuwa da gazawar HPLC da mafita
1, babban matsin lamba shine mafi yawan matsalar amfani da HPLC.Wannan yana nufin tashin matsi kwatsam.Gabaɗaya, akwai dalilai masu zuwa: (1) Gabaɗaya, wannan yana faruwa ne saboda toshewar tashoshi.A wannan gaba, ya kamata mu bincika shi gaba ɗaya.a.Da farko, yanke mashigan famfon.A wannan lokacin, bututun PEEK ya cika da ruwa ta yadda bututun PEEK ya yi ƙasa da kwalabe don ganin ko ruwan yana digowa yadda ya so.Idan ruwan ba ya digo ko digo a hankali, an toshe kan tace mai ƙarfi.Jiyya: Jiƙa a cikin 30% nitric acid na rabin sa'a kuma kurkura da ruwa mai ƙarfi.Idan ruwan ya faɗo ba da gangan ba, kan tace mai kaushi na al'ada ne kuma ana dubawa;b.Bude bawul ɗin Purge don kada tsarin wayar hannu ya wuce ta cikin ginshiƙi, kuma idan matsin lamba bai ragu sosai ba, an toshe farar mai tacewa.Jiyya: An cire fararen fata masu tacewa kuma an sonicated tare da 10% isopropanol na rabin sa'a.Tsammanin cewa matsa lamba ya faɗi ƙasa da 100PSI, fatar da aka tace ta al'ada ce kuma ana dubawa;c.Cire ƙarshen fita na ginshiƙi, idan matsa lamba bai ragu ba, an katange shafi.Jiyya: Idan yana da toshewar gishiri mai buffer, kurkura 95% har sai matsin ya zama al'ada.Idan wasu kayan da aka adana su ne suka haifar da toshewar, ya kamata a yi amfani da magudanar ruwa mai ƙarfi fiye da lokacin wayar hannu don gaggawar zuwa matsa lamba na yau da kullun.Idan matsa lamba mai tsafta na dogon lokaci bai ragu ba bisa ga hanyar da aka sama, ana iya la'akari da shigarwa da fitarwa na ginshiƙi don haɗawa da kayan aiki akasin haka, kuma za'a iya tsaftace ginshiƙan tare da tsarin wayar hannu.A wannan lokacin, idan har yanzu ba a rage matsi na shafi ba, za a iya maye gurbin farantin ƙofar ginshiƙi kawai, amma da zarar aikin ba shi da kyau, yana da sauƙi don haifar da raguwar tasirin shafi, don haka gwada amfani da ƙasa.Don matsaloli masu wahala, ana iya la'akari da maye gurbin shafi.
(2) Saitin ƙimar kwarara mara daidai: Ana iya sake saita madaidaicin ƙimar kwarara.
(3) Matsakaicin kwarara mara daidai: ma'aunin danko na ma'auni daban-daban na kwarara ya bambanta, kuma tsarin tsarin da ya dace na kwarara tare da danko mafi girma shima ya fi girma.Idan za ta yiwu, ana iya maye gurbin ƙananan ƙoƙon danko ko sake saitawa da shirya.
(4) Tsarin matsi na sifili: daidaita sifilin firikwensin matakin ruwa.
2, matsa lamba ya yi ƙasa sosai (1) yawanci yakan haifar da tsarin.Abin da za a yi: Nemo kowane haɗin gwiwa, musamman ma'amala a ƙarshen ginshiƙi, kuma ƙara ƙarar wurin ɗigogi.Cire post ɗin kuma ƙara ko daidaita fim ɗin PTFE tare da ƙarfin da ya dace.
(2) Gas yana shiga cikin famfo, amma matsa lamba yawanci ba shi da kwanciyar hankali a wannan lokacin, babba da ƙasa.Mafi mahimmanci, famfo ba zai iya ɗaukar ruwan ba.Hanyar jiyya: buɗe bawul ɗin tsaftacewa kuma tsaftace a cikin adadin 3 ~ 5ml / min.Idan ba haka ba, an sami kumfa na iska a cikin bawul ɗin shayewa ta amfani da bututun allura da aka keɓe.
(3)Babu fitowar lokaci ta wayar hannu: duba ko akwai lokacin wayar hannu a cikin kwalabe na tafki, ko nutsewa a cikin lokacin wayar, da kuma ko famfo yana gudana.
(4) Ba a rufe bawul ɗin tunani: an rufe bawul ɗin tunani bayan raguwa.Yawancin lokaci yana sauka zuwa 0.1.~ 0.2mL / min bayan rufe bawul ɗin tunani.
Taƙaice:
A cikin wannan takarda, matsalolin gama gari kawai a cikin chromatography na ruwa ana nazarin su.Tabbas, a aikace-aikacen mu, za mu ci karo da ƙarin wasu matsaloli.A cikin kula da kuskure, ya kamata mu bi ka'idodi masu zuwa: canza abu ɗaya kawai a lokaci guda don sanin alakar da ke tsakanin abin hasashe da matsalar;Gabaɗaya, lokacin da ake maye gurbin sassa don magance matsala, ya kamata mu mai da hankali ga mayar da ɓatattun sassan da aka tarwatsa don hana ɓarna;Ƙirƙirar ɗabi'ar rikodi mai kyau shine mabuɗin samun nasarar sarrafa kuskure.A ƙarshe, lokacin amfani da HPLC, yana da mahimmanci a kula da samfurin pretreatment da ingantaccen aiki da kiyaye kayan aiki.
Lokacin aikawa: Satumba 18-2023