Protein antigens na sake haɗawa sau da yawa suna da epitopes daban-daban, wasu daga cikinsu sune epitopes na jere wasu kuma na tsari ne.Magungunan rigakafi na polyclonal da aka samu ta hanyar rigakafi da dabbobi tare da antigens da aka lalatar da su sune gaurayawan rigakafi na musamman ga epitopes guda ɗaya kuma ana iya amfani da su gabaɗaya don gano sifofin halitta ko sunadaran da aka lalatar da su.Wani fa'idar yin amfani da sunadaran da ba su da ƙarfi a matsayin immunogens shine cewa sunadaran da ba su da ƙarfi sun zama mafi yawan rigakafi kuma suna iya haɓaka martani mai ƙarfi a cikin dabbobi.
Tsarin maganganun Escherichia coli yawanci ana zaba don dalilai na antigenic saboda shine tsarin mafi tsada dangane da lokaci da kuɗi.Don inganta yiwuwar bayyanar furotin da aka yi niyya da kuma dacewa da tsarkakewa, wani lokacin kawai an bayyana ƙananan guntu na furotin da aka yi niyya, kamar wani yanki na musamman.
Tsarin nau'i uku na sunadaran
Takamaiman Domain Na Protein
Idan manufar shirye-shiryen antibody shine kawai don gano wb, yana da tattalin arziki da sauri don amfani da ƙaramin peptide na roba azaman antigen, amma akwai haɗarin raunin immunogenicity ko rashin sakewa saboda zaɓin da bai dace na ɓangaren peptide ba.Tunda shirye-shiryen antibody yana buƙatar lokaci mai tsawo, ana zaɓi sassa biyu ko uku daban-daban na peptide don shirya ƙwayoyin rigakafi ta amfani da antigen polypeptide don tabbatar da ƙimar nasarar gwajin.
Ana buƙatar tsarkin antigen na polypeptide don rigakafi ya fi 80%.Ko da yake mafi girman tsarki na iya a zahiri samun ƙwayoyin rigakafi tare da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta, a aikace, dabbobi koyaushe suna samar da adadi mai yawa na ƙwayoyin rigakafi marasa takamaiman, don haka suna rufe fa'idodin tsarkakewar antigen.
Bugu da ƙari, shirye-shiryen ƙwayoyin rigakafi daga ƙananan peptides dole ne a haye su zuwa antigen mai ɗaukar nauyin da ya dace don haɓaka immunogenicity.Masu ɗaukar antigenic guda biyu na kowa sune KLH da BSA.
Lokacin aikawa: Maris 23-2023