A cikin binciken nazarin halittu, yawanci ana amfani da polypeptides tare da jerin dogon lokaci.Don peptides tare da amino acid sama da 60 a jere, ana amfani da maganganun kwayoyin halitta da SDS-PAGE gabaɗaya don samun su.Duk da haka, wannan hanya tana ɗaukar lokaci mai tsawo kuma sakamakon rabuwa na ƙarshe ba shi da kyau.
Kalubale da mafita don dogon kira na peptide
A cikin haɗuwa da dogon peptides, koyaushe muna fuskantar matsala, wato, stric stric rikitar da condensation dauki ya karu tare da karuwa da jerin a cikin kira, da kuma dauki lokaci bukatar a gyara domin mayar da martani cika.Duk da haka, tsawon lokacin amsawa, ana haifar da ƙarin sakamako masu illa, kuma an kafa wani ɓangare na peptide da ake nufi.Irin waɗannan ragowar - ƙarancin sarƙoƙin peptide sune mahimman ƙazanta waɗanda aka samar a cikin dogon kirar peptide.Don haka, a cikin haɗin dogon peptide, babbar matsalar da dole ne mu shawo kan ita ita ce bincika yanayin halayen halayen inganci da hanyoyin amsawa, ta yadda za mu sa amsawar amino acid ta zama cikakke kuma cikakke.Bugu da ƙari, rage lokacin amsawa, saboda tsawon lokacin amsawa, mafi yawan halayen da ba za a iya sarrafawa ba, mafi rikitarwa da samfurori.Don haka, an taqaice abubuwa guda uku kamar haka:
Ana iya amfani da haɗin microwave: Ga wasu amino acid da aka ci karo da su a cikin tsarin haɗin gwiwa waɗanda ba su da sauƙin haɗawa, ana iya amfani da haɗin microwave.Wannan hanyar tana da sakamako mai ban mamaki, kuma tana rage yawan lokacin amsawa, kuma tana rage samuwar samfuran maɓalli biyu.
Ana iya amfani da hanyar haɗin juzu'i: Lokacin da wasu peptides ke da wuya a haɗe su ta hanyoyin haɗin kai na gama gari kuma ba su da sauƙi don tsarkakewa, za mu iya ɗaukar duk nau'ikan amino acid da yawa a cikin wani yanki na peptide zuwa sarkar peptide gabaɗaya.Wannan hanyar kuma tana iya magance matsaloli da yawa a cikin haɗin gwiwa.
Ana iya amfani da kira na Acylhydrazide: acylhydrazide kira na peptides hanya ce ta m-lokaci kira na N-terminal Cys peptide da C-terminal polypeptide hydrazide sinadaran zabi dauki tsakanin samuwar amide bond don cimma peptide bonding hanya.Dangane da matsayin Cys a cikin sarkar peptide, wannan hanya ta raba dukkan sarkar peptide zuwa jeri da yawa kuma ta haɗa su bi da bi.A ƙarshe, ana samun peptide da aka yi niyya ta hanyar motsa jiki na lokaci-lokaci.Wannan hanya ba kawai rage girman lokacin kira na dogon peptide ba, amma har ma yana ƙara yawan tsarki na samfurin ƙarshe.
Dogon peptide tsarkakewa
Mahimmancin dogon peptides babu makawa yana haifar da hadaddun abubuwa na ɗanyen peptides.Saboda haka, yana da kuma ƙalubale don tsarkake dogon peptides ta HPLC.amyloid jerin polypeptide tsarkakewa tsari, sha da yawa kwarewa da kuma nasarar amfani da tsarkakewa na dogon peptide.Ta hanyar ɗaukar sabbin kayan aiki, haɗaɗɗun tsarin tsarkakewa da yawa, maimaitawa rabuwa da sauran hanyoyin gogewa, ƙimar nasarar dogon peptide tsarkakewa ya inganta sosai.
Lokacin aikawa: Afrilu-18-2023