Gyaran methylation

Methylation-gyaran peptides, kuma aka sani da methylation-gane peptides, su ne furotin bayan-fassara kayan ado (PTMs) kuma suna taka muhimmiyar rawa a cikin kusan dukkanin ayyukan rayuwa a cikin sel.Sunadaran suna da ƙarfi ta hanyar methyltransferase don canja wurin ƙungiyoyin hydroxyl zuwa takamaiman ragowar amino acid don ɗaure covalent.Methylation tsari ne mai jujjuyawa wanda demethylases ke haɓakawa.

Nazarin ya nuna cewa yawancin amino acid methylated/demethylated yawanci lysine (Lys) da arginine (Arg).Nazarin ya gano cewa histone lysine methylation yana da nau'o'in ayyuka na halitta kamar su kula da kwayoyin halitta da rarrabawa, X chromosome inactivation, tsarin rubutun da amsa lalacewar DNA.", yawanci yana rinjayar ƙumburi na chromatin kuma yana hana maganganun kwayoyin halitta."Histone arginine methylation yana taka muhimmiyar rawa a cikin tsarin rubutun kwayoyin halitta kuma yana iya rinjayar tsarin tsarin ilimin lissafi daban-daban a cikin sel, ciki har da gyaran DNA, fassarar siginar, ci gaban cell, da carcinogenesis.Saboda haka, Guopeptide Biology ya haɓaka fasaha na musamman na peptides na ado na methyl, wanda masana kimiyya suka gyara bayan fassarar furotin (PTMS) don taimakawa a cikin bincike.

Gyaran Methylation (Me1, Me2, Me3)

Babban ingancin Fmoc-Lys (Ni, Boc) -OH, Fmoc-Lys (Me2) -OH, Fmoc-Lys (Me3) -OH.HCL, Fmoc-Arg (Ni, Pbf) -OH, Fmoc-Arg (Ni) 2-OH.HCl (asymmetrical), F da aka yi amfani da moc-Arg (ni) 2-OH.HCl (symmetrical) da sauran albarkatun kasa da aka samar da FMOC m-lokaci kira tsari don samun Lys da Arg methylated peptides, da samfurori. HPLC ta tsarkake su.Abubuwan da suka dace na taron jama'a, HPLC chromatograms da COA ana ba da su don ƙãre samfurin.