Integrin, ko integrin, shine mai karɓar heterodimer transmembrane glycoprotein mai karɓa wanda ke daidaita mannewar kwayar halitta da sigina.Ya kunshiα kumaβ subunits.Yana da hannu a cikin haɓaka ayyukan salula daban-daban ciki har da ƙaurawar tantanin halitta, shigar da tantanin halitta, siginar tantanin halitta da intercellular, mannewar salula, da matakan angiogenesis.Integrinαvβ3 yanzu an fi bincikar su sosai.Bayyanar integrinαvβ3 yana da alaƙa da ƙaurawar ƙari, angiogenesis, kumburi da osteoporosis.Integrin yana bayyana sosai a cikin dukkanin ƙwayoyin ƙwayar cuta da kuma ƙwayoyin sel na endothelial na neovascularization.Bayyanar integrin yana da alaƙa da alaƙa da ƙaurawar ƙari da angiogenesis.A cikin 'yan shekarun nan, binciken kimiyya ya nuna cewa akwai integrins 11 waɗanda za su iya ɗaure musamman ga RGD peptide, waɗanda peptides ne na gaba ga masu karɓar integrin.
RGD peptide an kasafta shi cikin peptide RGD na layi da kuma RGD peptide cyclic.Idan aka kwatanta da peptide na RGD na layi, RGD cyclic peptide yana da karfin karfin karfin karba da kuma takamaiman mai karɓa.Wadannan sune nau'ikan gama-gari da hanyoyin haɗin kai na RGD cyclic peptide.
Nau'o'in gama-gari na RGD cyclic peptides:
1. Cyclic peptides dauke da jerin RGD kafa ta disulfide bonds
2. Cyclic peptides dauke da jerin RGD kafa ta amide bonds
Haɗin gwiwar RGD cyclic peptide:
Samfurin mai amfani yana da alaƙa da tsarin haɗin peptide na RGD cyclic a cikin fage na ingantaccen fasahar hada-hadar polypeptide.Sabuwar hanyar ita ce zabar 2-chloro-triphenylmethyl chloride resin a matsayin mai ɗaukar nauyi, da farko haɗa rukunin sarkar carboxyl na farko tare da rukunin kariya na musamman na D aspartic acid amino acid, sannan haɗa peptide madaidaiciya na jerin peptide na RGD zuwa guduro. , da kuma amino acid na ƙarshe don cire ƙungiyar kariya ta FMOC ba tare da piperidine ba.An ƙara ƙayyadadden mai haɓakawa don cire ƙungiyar kariya ta gefe na carboxyl na D aspartic acid na farko kai tsaye daga resin, sannan ƙari na piperidine don cire ƙungiyar kare amino FMOC na ƙarshen amino acid, sannan ƙari na wakili mai ɗauri. don rage ruwa da kuma tara ƙungiyar carboxyl da ƙungiyar amino da aka fallasa daga kai da ƙarshen peptide na layi kai tsaye daga resin a cikin hanyar amide bond don samar da peptide na cyclic.A ƙarshe, an yanke peptide na cyclic kai tsaye daga resin tare da maganin yanke.
Lokacin aikawa: Afrilu-24-2023