Dangane da hanyar haɗin amino acid da sukari, ana iya raba peptide na sukari zuwa rukuni huɗu: O glycosylation, C a N glycosylation, dew saccharification da GPI (glycophosphatidlyinositol).
1. N-glycosylation glycopeptides suna kunshe da N-acetamide glucose a rage ƙarshen sarkar glycan (Glc-Nac) da ke da alaƙa da N atom akan rukunin amide na sashin gefe na wasu Asn a cikin sarkar peptide, da kuma Asn. wanda zai iya haɗa sarkar glycan dole ne a kasance a cikin AsN-X-Ser / Thr (X! = P) a cikin maƙasudin kafa ta ragowar.Sugar shine N-acetylglucosamine.
N-glycosylation gyara tsarin glycopeptide
2. Tsarin O-glycosylation ya fi sauƙi fiye da N-glycosylation.Wannan glycopeptide gabaɗaya ya fi glycan gajeru, amma yana da nau'ikan fiye da N-glycosylation.Ser da Thr na iya zama glycosylated gabaɗaya a cikin sarkar peptide.Bugu da ƙari, akwai glycopeptides da aka yi wa ado da tyrosine, hydroxyl, da hydroxyproline glycosylation.Matsayin haɗin kai shine atom ɗin oxygen na hydroxyl akan sashin gefen ragowar.Abubuwan da ke da alaƙa sune galactose ko N-acetylgalactosamine (Gal&GalNAc) ko glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), da sauransu.
O-glycosylation yana gyara tsarin
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
N-acetylglucosamine guda ɗaya (GlcNAc) glycosylation yana haɗa sunadaran O-GlcNAc zuwa atom ɗin oxygen na hydroxyl na serine ko ragowar threonine na furotin.O-GlcNA glycosylation shine O-GlcNAc monosaccharide kayan ado ba tare da tsawo na glycan ba;Kamar peptide phosphorylation, O-GlcNAc glycosylation na glycopeptides shima tsari ne na kayan ado na furotin.Kyawawan ado na O-GlcNAc na iya haifar da cututtuka iri-iri kamar su ciwon sukari, cututtukan zuciya, ciwace-ciwace, cutar Alzheimer da sauransu.
Glycosylation maki na glycopeptides
Sifofin asali na polypeptide da sarƙoƙin sukari suna da alaƙa da sarƙoƙin furotin ta hanyar haɗin gwiwa, kuma rukunin yanar gizon da ke haɗa sarƙoƙin sukari ana kiran su wuraren glycosylation.Tun da babu samfuri don bin biosynthesis na sarkar sukari na glycopeptide, za a haɗa sarƙoƙin sukari daban-daban zuwa rukunin glycosylation iri ɗaya, wanda ke haifar da abin da ake kira inhomogeneity microscopic.
Glycosylation na glycopeptides
1. Tasirin glycopeptide glycosylation akan jiyya-tasirin sunadaran warkewa.
A cikin yanayin jiyya-maganin furotin, glycosylation kuma yana shafar rabin rayuwa da niyya na magungunan furotin a cikin vivo.
2. Soluble glycopeptide glycosylation da sunadarai
Nazarin ya nuna cewa sarƙoƙin sukari a saman sunadaran suna iya inganta haɓakar ƙwayoyin sunadarai
3. Glycopeptide glycosylation da furotin immunogenicity
A gefe guda, sarƙoƙin sukari a saman sunadaran suna iya haifar da takamaiman martani na rigakafi.A gefe guda kuma, sarƙoƙi na sukari na iya rufe wasu filaye akan saman furotin kuma su rage rigakafi
4. Glycopeptide glycosylation wanda ke kara karfin furotin
Glycosylation na iya ƙara kwanciyar hankali na sunadaran zuwa yanayi daban-daban na denaturation (kamar denaturants, zafi, da dai sauransu) da kuma guje wa tarukan sunadaran.A lokaci guda kuma, sarƙoƙin sukari a saman sunadaran suna iya rufe wasu abubuwan lalata ƙwayoyin furotin, ta haka ne ke haɓaka juriyar sunadaran ga furotin.
5. Glycopeptide glycosylation wanda ke shafar ayyukan nazarin halittu na kwayoyin sunadarai
Canza glycosylation sunadaran kuma na iya ba da damar ƙwayoyin furotin su samar da sabbin ayyukan nazarin halittu
Lokacin aikawa: Agusta-03-2023