Tsarin ƙira da maganin sarkar peptide polypeptide

I. Taƙaice
Peptides su ne macromolecules na musamman waɗanda jerin su ba sabon abu bane a cikin sifofinsu na sinadarai da na zahiri.Wasu peptides suna da wahalar haɗawa, yayin da wasu suna da sauƙin haɗawa amma suna da wahalar tsarkakewa.Matsala mai amfani ita ce yawancin peptides suna da ɗan narkewa a cikin mafita mai ruwa, don haka a cikin tsarkakewarmu, dole ne a narkar da sashin da ya dace na peptide hydrophobic a cikin abubuwan da ba na ruwa ba, Saboda haka, waɗannan kaushi ko buffers suna iya zama mai tsanani da rashin daidaituwa tare da amfani. na hanyoyin gwaji na nazarin halittu, ta yadda masu fasaha an hana su yin amfani da peptide don dalilai na kansu, ta yadda waɗannan abubuwa ne da yawa na ƙirar peptides ga masu bincike.

Tsarin ƙira da maganin sarkar peptide polypeptide
Na biyu, daidai zabi na roba wuya peptides
1. Jimlar tsawon jerin abubuwan da aka tsara
Peptides na ƙasa da ragowar 15 sun fi sauƙi a samu saboda girman peptide yana ƙaruwa kuma tsabtar ɗanyen samfurin yana raguwa.Yayin da jimlar sarkar peptide ke ƙaruwa sama da saura 20, ainihin adadin samfurin shine babban abin damuwa.A cikin gwaje-gwaje da yawa, yana da sauƙi don samun tasirin da ba zato ba tsammani ta rage ragowar lambar ƙasa 20.
2. Rage yawan ragowar hydrophobic
Peptides tare da babban rinjaye na ragowar hydrophobic, musamman a cikin yanki na 7-12 saura daga C-terminus, yawanci suna haifar da matsalolin roba.Ana ganin wannan a matsayin haɗin da bai dace ba daidai saboda ana samun takardar B-fold a cikin haɗin."A irin waɗannan lokuta, yana iya zama da amfani don canza fiye da saura masu kyau da mara kyau, ko sanya Gly ko Pro a cikin peptide don buɗe abun da ke cikin peptide."
3. Downregulation na "wuya" saura
"Akwai adadin Cys, Met, Arg, da Gwada ragowar da ba a haɗa su cikin sauri."Yawanci za a yi amfani da Ser a matsayin madadin marasa iskar oxygen zuwa Cys.
Tsarin ƙira da maganin sarkar peptide polypeptide

Na uku, inganta ingantaccen zaɓi na mai narkewa a cikin ruwa
1. Daidaita tashar N ko C
Dangantaka da peptides acidic (wato, ana cajin da ba daidai ba a pH 7), acetylation (N-terminus acetylation, C terminus koyaushe yana riƙe ƙungiyar carboxyl kyauta) ana ba da shawarar musamman don ƙara ƙarancin cajin.Koyaya, don ainihin peptides (wato, ana cajin su a pH 7), amine (ƙungiyar amino kyauta a N-terminus da amination a C-terminus) ana ba da shawarar musamman don ƙara ingantaccen cajin.

2. Gajarta sosai ko tsawaita jerin

Wasu daga cikin jerin suna ɗauke da adadi mai yawa na amino acid hydrophobic, irin su Trp, Phe, Val, Ile, Leu, Met, Tyr da Ala, da dai sauransu. Lokacin da waɗannan ragowar hydrophobic suka wuce kashi 50%, yawanci ba su da sauƙin narkewa.Yana iya zama da amfani don tsawaita jerin don ƙara haɓaka ingantattun sanduna masu kyau da mara kyau na peptide.Zaɓin na biyu shine rage girman girman sarkar peptide don ƙara haɓakar sanduna masu kyau da mara kyau ta hanyar rage ragowar hydrophobic.Ƙarfafa ɓangarorin tabbatacce da mara kyau na sarkar peptide, mafi kusantar shi shine amsawa da ruwa.
3. Saka a cikin ragowar ruwa mai narkewa
Ga wasu sarƙoƙin peptide, haɗewar wasu amino acid masu kyau da marasa kyau na iya inganta narkewar ruwa.Kamfaninmu yana ba da shawarar N-terminus ko C-terminus na peptides acidic don haɗa su da Glu-Glu.An ba da ƙarshen N ko C na asali na peptide sannan Lys-Lys.Idan ba za a iya sanya ƙungiyar da aka caje ba, Ser-Gly-Ser kuma ana iya sanya shi a cikin tashar N ko C.Duk da haka, wannan hanya ba ta aiki lokacin da ba za a iya canza sassan sarkar peptide ba.