Cortistatin-29 (bera)/1815618-17-7/GT Peptide/Mai ba da Peptide

Bayani

Corticostatin 29 neuropeptide ne wanda yake da tsari mai kama da somatostatin 28. 1 Ana samar da shi ta hanyar ɓarkewar procortatin zuwa procortatin, wanda ke sharewa a cikin amino acid na binary don samar da cortatin 29 da cortatin 14 da sauran samfuran cleavage.An bayyana Corticostatin mRNA a cikin kwakwalwar ɗan adam da kuma a cikin interneurons a cikin hippocampus da cerebral cortex na berayen.1, 2 Cortistatin-29 an ɗaure zuwa mai karɓar somatostatin (SST) tare da ƙimar IC 50 na 2.8, 7.1, 0.2, 3 da 13.7 nM don SST 1-5, bi da bi.2 ya sami irin wannan matakan corstatin 29 zuwa corstatin 14 a cikin ƙwayoyin linzamin kwamfuta na AtT20, amma a ƙananan matakan ɓoye.Cortistatin-29 yayi daidai da ragowar 85 zuwa 112 na jerin peptide na bera.

Ƙayyadaddun bayanai

Apperance: Fari zuwa kashe-fari foda

Tsarki (HPLC):≥98.0%

Tsaftace Guda Daya:≤2.0%

Abun cikin Acetate (HPLC): 5.0%～12.0%

Abubuwan Ruwa (Karl Fischer):≤10.0%

Abun cikin Peptide:≥80.0%

Shiryawa da jigilar kaya: Ƙananan zafin jiki, shiryawar injin, daidai zuwa MG kamar yadda ake buƙata.

Yadda Ake Oda?

1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.

2. Yi oda akan layi.Da fatan za a cika tsari na kan layi.

3. Samar da sunan peptide, CAS No. ko jerin, tsabta da gyare-gyare idan an buƙata, yawa, da dai sauransu za mu samar da zance a cikin 2 hours.

4. oda conformation ta duly sa hannu tallace-tallace kwangila da kuma NDA(ba bayyana yarjejeniya) ko sirri yarjejeniya.

5. Za mu ci gaba da sabunta tsarin ci gaba a cikin lokaci.

6. Bayar da Peptide ta DHL, Fedex ko wasu, da HPLC, MS, COA za a ba su tare da kaya.

7. Za a bi manufar mayar da kuɗi idan wani rashin daidaituwa na ingancinmu ko sabis ɗinmu.

8. Bayan-sale sabis: Idan mu abokan ciniki suna da wasu tambayoyi game da mu peptide a lokacin gwaji, don Allah ji free tuntube mu kuma za mu amsa da shi a cikin wani gajeren lokaci.

Duk samfuran kamfanin ana amfani da su ne kawai don dalilai na bincike na kimiyya, shi's an haramta yin amfani da shi kai tsaye da kowane mutum a jikin ɗan adam.

FAQ:

Shin peptides ɗin da ke ɗauke da Cys an rage kafin jigilar kaya?

Idan ba a sami peptide ya zama oxidized ba, gabaɗaya ba mu rage Cys ba.Ana samun duk polypeptides daga samfuran da aka tsarkake da lyophilized a ƙarƙashin yanayin pH2, wanda aƙalla ya hana iskar oxygenation na Cys.Peptides dauke da Cys ana tsarkake su a pH2 sai dai idan akwai takamaiman dalili don tsarkakewa a pH6.8.Idan ana yin tsarkakewa a pH6.8, samfurin da aka tsarkake dole ne a bi da shi tare da acid nan da nan don hana iskar oxygenation.A mataki na kula da inganci na ƙarshe, don peptides da ke ɗauke da Cys, idan an sami kasancewar nauyin kwayoyin halitta (2P + H) akan taswirar MS, yana nuna cewa an kafa dimer.Idan babu matsala tare da MS da HPLC, za mu kai tsaye lyophilize da jigilar kaya ba tare da wani aiki ba.Ya kamata a lura cewa peptides dauke da Cys suna yin jinkirin oxidation na tsawon lokaci, kuma matakin oxidation ya dogara da jerin peptide da yanayin ajiya.

Ta yaya za ku tantance idan an madauki peptide?

Muna amfani da amsawar Ellman don gwada ko samuwar zoben ya cika.Idan gwajin Ellman tabbatacce ne (rawaya), yanayin zoben bai cika ba.Idan sakamakon gwajin ya kasance mara kyau (ba rawaya ba), amsawar zoben ya cika.Ba mu bayar da rahoton bincike na gano keken keke ga abokan cinikinmu ba.Gabaɗaya, za a sami bayanin sakamakon gwajin Ellman a cikin rahoton QC.

Ina bukatan peptide na cyclic, wanda ya ƙunshi tryptophan, zai zama oxidized?

Rashin iskar oxygen na tryptophan abu ne na kowa a cikin peptide oxidation, kuma peptides yawanci ana yin hawan keke kafin tsarkakewa.Idan hadawan abu da iskar shaka na tryptophan ya faru, lokacin riƙewar peptide a kan ginshiƙi na HPLC zai canza, kuma ana iya cire iskar oxygen ta hanyar tsarkakewa.Bugu da ƙari, ana iya gano peptides oxidized ta MS.

Shin wajibi ne a sanya rata tsakanin peptide da rini?

Idan za ku haɗa babban kwayoyin halitta (kamar rini) zuwa peptide, yana da kyau a sanya sarari tsakanin peptide da ligand don rage tsangwama tare da mai karɓa ta hanyar nadewa peptide kanta ko ta nadewa. ta conjugate.Wasu ba sa son tazara.Misali, a cikin nadawa sunadaran, ana iya tantance nisan tsarin nadawa na amino acid ta hanyar makala rini mai kyalli zuwa wani wuri.

Idan kuna son yin gyaran biotin a tashar N, kuna buƙatar sanya rata tsakanin biotin da jerin peptide?

Daidaitaccen tsarin alamar biotin da kamfaninmu ke amfani da shi shine haɗa Ahx zuwa sarkar peptide, sannan biotin ya biyo baya.Ahx wani fili ne mai 6-carbon wanda ke aiki azaman shamaki tsakanin peptide da biotin.

Shin za ku iya ba da shawara kan ƙirar peptides phosphorylated?

Yayin da tsayin ya ƙaru, ƙimar ɗaurin a hankali yana raguwa daga amino acid phosphorylated zuwa gaba.Jagoran kira yana daga tashar C zuwa tashar N.Ana ba da shawarar cewa ragowar amino acid na phosphorylated kada su wuce 10, wato adadin amino acid kafin amino acid na phosphorylated daga tashar N zuwa tashar C kada ya wuce 10.

Me yasa n-terminal acetylation da C-terminal amidation?

Waɗannan gyare-gyare suna hana peptide daga lalacewa kuma suna ba da damar peptide ya yi kama da ainihin yanayin alpha amino da ƙungiyoyin carboxyl a cikin furotin iyaye.